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GCHPLCLiquid measurementMass spectrometryMetAmino KitSPE & MSPE

Application lists

2

2-Formylbenzenesulfonic acid in Zinc plating baths (APK #498)

Chemical additives are widely used in electroplating technology. This application shows the method of HPLC for determination of 2-formylbenzenesulfonic acid, which is used as additive in Zinc plating baths.

A

Acetylsalicyclic acid (APK #317)

Acetylsalicylic acid is very well-known as the drug Aspirin and is used to reduce pain and fever. It is also supplied under other brand names, such as Acylpyrin or Anopyrin. This application note shows the determination of impurity – salicyclic acid in the final product.

Additives in plastics (#338)

Polymer additives are important for stabilization in manufacturing process and stabilization over time. This application note shows the analysis of selected a process, UV and oxidation stabilizers widely used in polymers. The samples are extracted under reflux and after polymer precipitation, the additives are analyzed by HPLC. The results are essential for quality control.

Aflatoxins and ochratoxin by HPLC/FLD (APK #344)

Aflatoxin is a Group 1 carcinogen and is a product of mould. Ochratoxin is a mycotoxin produced by the fungus Aspergillus ochraceous and also by several species of Penicillium fungi. It is considered a potential carcinogen and has been known to cause kidney damage. All these mycotoxins are monitored worldwide and allowed concentration limits depend not only on the territory, but also on the food/feed matrix and are given by local administrations.

Aflatoxins by LC/MS (APK #343)

Aflatoxins are Group 1 carcinogens and are a natural product of mould. Mycotoxines are monitored worldwide and allowed concentration limits depend not only on the territory, but also on the food/feed matrix and are given by local administrations.

Alcaloids - Xanthine derivatives (APK #293)

Xanthine alkaloids occur naturally in various plants, such as cocoa, tea and coffee tree. They are commonly used for their effects as mild stimulants. Xanthine alkaloids are monitored in food and drinks, e.g. in chocolate, cocoa powder, and energy drinks.

Amines (APK #458)

This application shows very nice peek symmetry and separation of aromatic amines in a Engelhardt mixture.

Amines and aromatics (APK #378)

Amines is a group of compounds, which is not easy to separate. The Novel ASTRA® DM phase with a polar embeded group and polar selector enables the separation of different amines and organic compounds with an amino-functional group. Peaks show an excellent symmetry under shown conditions.

Amino Acid Analysis in Fish Feed (APK #530)

Monitoring the concentration of amino acids in fish feed is critical to the health, growth and sustainability of aquaculture operations for several reasons. Firstly, fish feed should be rich in proteins, which are essential for fish growth and development. Further, certain amino acids play a crucial role in maintaining and enhancing the fish’s immune system, which helps them resist disease and reduce mortality rates. Monitoring ensures that fish feed contains all essential amino acids in the right proportions to avoid nutritional deficiencies that can lead to poor growth, deformities and increased susceptibility to disease.

Amino acids analysis in beer (APK #497)

The flavor diversity in beer strongly influenced by amino acids and proteins originating from the raw materials and the technological processes of beer brewing. Using our LC-MS MetAmino® Kit, we tested a sample of Budvar beer. Here we present a comparison of the mass chromatograms of the standard mixture and Budvar 12˚ beer.

Amino acids analysis in human blood serum (APK #506)

Amino acids play an important role in the life processes of human organism. They are using as building blocks for proteins and regulate a key metabolic pathways. Many kinds of mental disorders, liver and immune system diseases are related to metabolic disorders of amino acids. AAs profile, determined in human blood serum, is used for an early diagnostic of various diseases. This application shows amino acids profile in healthy human blood serum tested by LC-MS MetAmino® kit.

Amino acids analysis in human urine (APK #505)

Amino acids analysis in urine is excellent tool for identification of gastrointestinal and renal dysfunctions. It also may provide supportive information towards nutritional deficiencies of vitamins and minerals. MetAmino® kit provides a rapid, simple and precise method for the quantitative determination of wide panel of amino acids in urine. The test reflects recent diet and may be used to assess the impact of dietary changes.

Amino acids analysis in Tilapia sperm (APK #501)

Amino acids plays a valuable role in spermatogenesis, fertilization success and impactson survival of juvenile fish. This application shows amino acids profile in Tilapia sperm tested by LC-MS MetAmino® kit. The data give better understanding of adjustment in feeding ration needed to increase reproductive function and population of food fish species.

Amitraz in Varidol (APK #346)

The Beekeeping Research Institute produces Amitraz solution in concentration 125 mg/l, which is used as an insecticide. ARION® Plus C18 shows much less contamination by final product, which includes a heavy matrix (aromatic naphtha fraction). Even after many analyses the chromatogram shows a peak with a high symmetry/tailing factor (1.019).

Ampicillin in human blood plasma (APK #409)

The accurate, simple, and selective HPLC/UV method has been established and validated for the determination of the antibiotic AMP as a suitable method in a cardiac surgery which does not have to be used only in cardiac surgery. The new ARION® Polar C18 column has been shown to effectively separate AMP in human blood plasma.

Analysis of Anti-infective agents (APK #557)

Anti-infectives are a class of many types of drugs that cover a broad range of infections, including antibiotics, antifungals, antiviral, and even protozoal infections. They have different mechanisms of action based on pathogens transmission and interaction with their host. Column ASTRA® allows to separate a broad spectrum of anti-infectives with a completely different chemical structure.

Analysis of drugs of abuse (APK #560)

Drug abuse has become a serious problem worldwide. Determination of drugs of abuse is important for undertanding of the needed treatment as well as for the law enforcement. The list of target compounds to be screened can number in the hundreds. This application shows a rapid analysis of the drugs of abuse belonged to different classes.

Analysis of long-chain PFASs in biological sample matrix (APK #533)

PFASs are a group of synthetic organofluorine chemical compounds used in the production of fluoropolymer coatings and products such as furniture, adhesives, personal care products, food packaging, mobile phone screens, etc. Many PFASs pose health and environmental concerns because they are classified as persistent organic pollutants. The long-chain PFASs are due to higher bioaccumulative potential frequently detected in living organisms. This application shows a robust method of extraction and determination of the PFAS compounds in fish biota samples, which can be a relevant exposure pathway for humans.

Analysis of sugars (APK #537)

The analysis of saccharides and sugar alcohol is one of the most common criteria in food and beverage analyses in the QC laboratories of manufacturers and the subject of monitoring from authorities. This analysis is also used to detect food and beverage adulteration.

Antidiabetic drugs (APK #564)

Diabetes is a chronic condition that occurs when the body cannot produce any or enough insulin, or cannot effectively use the insulin it produces. Type 2 diabetes has attained the status of a global pandemic. Sulfonylureas represent a class of medications used in the treatment of type 2 diabetes mellitus. All sulfonylureas contain a phenyl-sulfonyl-urea structure, which exerts the hypoglycemic effect. An efficient and reliable LC/MS method has been developed for сontrol of antidiabetic
medications of sulfonylurea class glibenclamide and glimepiride.

Antioxidants in polyolefin granulate (APK #374)

The antioxidants/UV stabilizers in the polyolefin granulate are analyzed by the standard addition method. These polymers are used as a catalytic media during crosslinking and further processing.

API glucuronide by LC/MS (APK #372)

This application shows high ASTRA® HPLC column stability in the pharmacokinetic assay. API is used in hypolipidemic therapy adjusting cholesterol levels in the human body.

Astaxanthin (APK #566)NEW

Astaxanthin is a lipid soluble pigment with а proven antioxidative properties. It is used as a nutritional supplement for human, in cosmetics and as a feed additive to impart coloration for salmon, crabs, shrimp and other aquatic organisms. A major source for the biotechnological production of astaxanthin is the green microalgae. The astaxanthin measurement is needed during the algae cultivation process as well as at astaxanthin extraction stage. A precise chromatographic method has been developed for astaxanthin quantification.

Atorvastatin and photodegradation products (APK #406)

The HPLC method with UV and FLD detection has been optimized for the evaluation of photochemical degradation kinetics of atorvastatin in aqueous solutions under light conditions relevant to natural waters. Knowledge of the relative proportion of atorvastatin and its degradation products in water solution is needed for the further study of a potential toxic effect of atorvastatin itself and of the mixture of products of atorvastatin photochemical degradation in a water environment.

ATP Metabolites (APK #358)

This application note has been developed by the Institute of Organic Chemistry and Biochemistry, the group of synthesis of radiolabeled compounds. It shows separation of nucleosides, and nucleoside phosphates that are analysed in different applications, e.g. purine metabolism, biosynthesis, genetic code and clinical studies.

Avenanthramides (APK #326)

Avenanthramides (AVN) are phenolic alkaloids that are found mainly in oats. They are used in various diary supplements and dermatology products thanks to their anti-inflammatory effects and antioxidant activities.

Azithromycin (APK #329)

Azithromycin is an antibiotic used to treat many different types of infections. It belongs to a group called macrolides and has relatively broad antibacterial activity.

B

Beta Blockers (APK #371)

Beta Blockers are also known as beta-adrenergic blocking agents. They are used as a medication that reduces blood pressure.

Bitter acids in hop (APK #311)

Alpha-bitter acids are precursors of iso-α-bitter acids that are formed during the brewing process. They are present in hops (Humulus Lupulus L.) and their content depends on plant species and growing conditions. Iso-α-bitter acids give an appreciated bitter taste to the beer.

C

Caffeine metabolites (APK #401)

The HPLC/UV method was developed, optimized and validated for the analysis of caffeine and its selected metabolites (1-methyluric acid, theobromine, paraxanthine, theophylline) in urine. The method is also used for the quantification of caffeine in various food samples.

Calcium gluconate in injection solution (APK #474)

Calcium is found naturally in the body and is necessary for the normal function of muscles and nerves. It is needed to make the heart work properly and for blood to clot. In injections it is used in the form of calcium gluconate salt. The presented method shows the in-process control of calcium gluconate and stabylizer calcium saccharate in injection solution.

Cannabinoids (APK #310)

Cannabinoids have became more and more popular thanks to their health effects and the decriminalisation of their use. Analytical columns that can offer a suitable resolution play an important role. The challenge is to achieve the separation of the critical pair – CBD and CBG.

Cardiovascular drugs (APK #559)

A gradient LC/MS method was developed for rapid determination of several classes of cardiovascular drugs such as non-cardioselective beta blockers, potassium-sparing diuretics, statins, nucleoside transport inhibitors, calcium channel blockers, ACE inhibitors, ARBs, lipid-lowering agents and anticoagulants.

Cefazolin sodium (APK #453)

Cephalosporin antibiotic cefazolin sodium is widely used in medicine practice. ARION® Polar C18 column provides perfect resolution factor and peaks symmetry according to the pharmacopoeial method for determination of cefazolin sodium.

Ceramides (APK #484)

Ceramides are a family of fatty acids molecules. They play a role in skin’s barrier function and also promote brain development and maintain cell function. They are widely used as a moisturizer in various cosmetic and personal products. The application shows a sensitive LC/MS method for fast determination of diverse group of ceramides.

CIT/MIT preservative determination

CIT/MIT biocides are widely used as preservatives in variety of applications, which include household products, personal care products and polymer materials. Apart of their antimicrobial activity, CIT/MIT preservatives may cause allergy reactions. Due to this reason, the preservatives additives should be used at the lowest possible concentration and strictly controlled. This application shows the determination of CIT/MIT preservative in rhamnolipids production. ARION® C8 column shows good resolution and peaks symmetry for CIT/MIT components at low concentrations.

Citrates in Infusion Solution (APK #370)

Citrate is a chemical stabilizer used in infusion solutions. However, infusion of citrate is responsible for a decrease of arterial blood preessure. So, it is very important to keep optimum concentration in citrate infusion.

Clonazolam (APK #381)

Xanax is an benzodiazepine alprazolam-based anxiolitic. Counterfeit Xanax may contain caffeine as a main component, as well as clonazolam and flualprazolam, both of which have a strong sedative effect, are able to cause amnesia, and belong to the group of new psychoactive drugs.

CNS agents monitoring (APK #563)

There are many different types of drugs that affect the central nervous system (CNS), including anesthetics, anticonvulsants, antiemetics, antiparkinson agents, CNS stimulants, muscle relaxants, narcotic analgesics and sedatives. Their control is important for therapeutic monitoring and in toxicologic purposes. The developed LC/MS method provides the accurate determination of a wide range of CNS agents.

Codeine phosphate (APK #466)

Control of active pharmaceutical ingredients should meet the strict requirements of pharmacopeial monographs. ARION® Plus C18 column provides perfect tailing
factor and resolution coefficient according to USP Monograph for codeine phosphate in comparison with competitive’s column.

Confident identification of unknown new psychoactive substances by a unique software workflow

Constant innovation in the creation of new psychoactive substances (NPS) means that new, potentially harmful substances are frequently entering the market, making it crucial to stay ahead in identification. The ability to confirm unknown chemical identities quickly and accurately is critical for law enforcement, security, and public safety. High-resolution tandem mass spectrometry (HR-MS/MS) and innovative data processing offer detailed insights into known drug targets and the detection of unexpected compounds. This study employs MetaboScape, a specialized software, for nontarget processing of HR-MS/MS data, facilitating the identification of NPS in seized powders. MetaboScape’s optimized workflow covers elemental formula calculation and structural confirmation, ensuring robust compound identification. The study showcases MetaboScape’s effectiveness in identifying Modafiendz, a fluorinated analog of the controlled substance modafinil, in a seized powder.

D

Degradation assessment of diltiazem (APK #407)

The HPLC/UV method has been optimized for the degradation assessment of calcium channel blocker diltiazem. The photochemical degradation kinetics of this benzodiazepine structure is studied in aqueous solution.

Denatonium benzoate in ethanol (APK #294)

Denatonium benzoate is considered the most bitter compound by the whole world, which is why it is used as a denaturant of ethanol to prevent its misuse. This application shows fast isocratic elution to enhance productivity in the laboratory.

Detection of PFOS pollutants

Environmental water screenings are regularly challenged by the number and diversity of chemical and biological targets in a given sample. Traditional chromatographic separation can be insufficient to discriminate between closely related compounds or meet established detection limits. The combination of the Bruker TargetScreener LC method with trapped ion mobility spec­trometry via the Bruker timsTOF Pro system enables rapid discrimination of PFOS pollutants, including isomers, with significantly improved target detection sensitivity.

Determination of aromatic hydrocarbons in diesel fuel (APK #519)

The aromatic hydrocarbons content of diesel fuel is a factor that can affect exhaust emissions and fuel combustion characteristics, as measured by cetane number. This application shows fast and robust method for the determination of monoaromatic, di-aromatic, and polyaromatic hydrocarbon contents in diesel fuels and petroleum distillates boiling in the range from 150 to 400 °C.

Dihydrotestosterone (APK #478)

Dihydrotestosterone (DHT) is an androgenic hormone synthesized primarily in prostate. DHT plays a primary role in the formation of the male genitalia during embryogenesis and it acts as the primary androgen in the prostate and hair follicles in adulthood. In medicine it is used mainly in the treatment of low testosterone levels. ARION® Biphenyl column allows to determine DHT with sufficient tailing factor and resolution coefficient in the complex mixture of excipients in finished dosage form.

Dissolution profile pyridoxine and benfotiamine (APK #418)

The development of the HPLC method for vitamin mixtures in drug products is always challenging, taking into account the requirements of analytical methods in pharmaceutical production. Shown below is a chromatogram of dissolution profile of tablets that contain a mixture of pyridoxine hydrochloride and benfotiamine. ARION® Polar C18 column provides a perfect resolution factor and peaks symmetry in a long sequence of analysis.

E

End-group identification of structural isomeric polymers

MALDI mass spectrometry provides highly specific chemical information for polymer structural analysis, copolymer composition, complex polymer mixtures, and surface imaging, making it invaluable in various polymer analysis applications. This technique is widely recognized for the characterization of synthetic polymer samples and is used to calculate average molecular weights and identify end-groups. In this application note, polyethylene glycol methacrylate, methyl ether acrylate, and diglycidyl ether, which are structural isomers with equal oligomer masses, were identified by MALDI-CID-MS/MS. End-group analysis by MS is based on the total mass of both end-groups, but MS/MS has the advantage of being able to identify each end-group individually.

Ergot alkaloids (APK #444)

Ergot alkaloids are mitotoxins produced by several species of fungies. Many different cereals may become contaminated with ergot alkaloids that cause poisoning in human and animals is known as ergotism. Despite on actions taken in agricultural practice for grain purification, control of the ergot alkaloids is still actual taking into account the low toxicological limits of these mitotoxins.

F

FAME (APK #388)

Fatty acids are carboxylic acids with a long side carbon chain typically found in lipids. These acids differ by the number of carbon atoms in the chain and the number of double bonds in the chain. According to the number of double bonds we distinguish saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). Trans fatty acids are unsaturated fatty acids in which at least one double bond is in the trans position. Capilary column LION™ LN-FAME was designed to provide the required polarity by the high-cyano propyl phase (G48). In this application note you can see a fast, robust and reproducible baseline separation of the 37 most common FAMEs.

Fast adulteration testing of expensive food ingredients by DART-HRMS: example for truffles

Food fraud is a major issue in the food industry leading to financial losses for food processors, inflicting lasting damage in the trust of consumers and in the most severe cases even threatening public health. The combination of DART with QTOF-MS and the software package MetaboScape is a comprehensive solution for food quality control as shown in this example for black truffles. Truffles are considered a luxury product, consequently, there is a concern about adulteration, which can encompass blending authentic truffles with lower-quality alternatives or employing artificial flavorings to replicate the truffle taste. Aside of just classification, the method enables the identification of possible markers down to a detailed elucidation of the underlying molecular structures. A discrimination between the different black truffle species was achieved by unsupervised and supervised statistical models. Distinctive marker compounds were identified and annotated based on their accurate mass, isotope pattern and fragmentation pattern. Analysis of one sample is finished in 15 seconds, the chromatography-free workflow highly reduces solvent consumption and limits exposure of operators to toxic solvents.

Fatty acids (APK #457)

Control of the profile of fatty acids in blood samples is actual due to the role of fatty acids as biomarkers in the pathogenesis of atherosclerosis. Capillary Column LION™ LN-FAME HT GC provides separation of the 40 fatty acids with excellent reproducibility and robustness.

Flavonoids and their sulfated metabolites by HPLC method (APK #462)

Flavonoids have antioxidant and antiviral properties and are widely used as drugs and dietary supplements. Sulfation reaction of flavonoid compounds is part of mammalian metabolism. The presented method allows to separate parent compounds luteolin, quercetin, silychristin and their sulfated derivates in enzymatic sulfation reaction mixtures.

Food supplements (APK #473)

Food supplements are now trending worldwide. Concentrated sources of nutrients, vitamins and aminoacids are used to support the normal nutritional balance and healthy lifestyle. This application shows the simultaneous determination of cAMP, phenylalanine, tryptophan, FAD, malic and citric acids by accurate HPLC method.

Furans in transformer oil (APK #315)

Furans analysis together with oil soluble metal deactivator is an important analysis to monitor the degradation of winding insulation in the transformers. The presence of furans in transformer oils show the stage of the insulation degradation and a need of transformer replacement.

H

Herbicides by LC/MS (APK #357)

This application shows the separation of selected herbicides from the picolinic acid and phenoxyacetic acid family.

HMF in Infusion Solution (APK #369)

The levels of the degradation product, 5-hydroxymethylfurfural (5-HMF), in Dextrose Injection is an important determination in pharmaceutical formulas and pharmacokinetic studies. ASTRA® DM shows better retention for polar compounds.

HMF in Syrup (APK #312)

5-Hydroxymethylfurfural (5-HMF) is formed from fructose or glucose by the heat treatment of food. HMF and its derivates/metabolites are genotoxic, mutagenic and may be carcinogenic, which is why HMF is analysed in various food matrices, such as in fruit and vegetable products, instant coffee and honey.

Hormones receptors agents (APK #565)

Hormones are responsible for carrying out various functions in the human body. A treatment of lot of diseases is based on hormones receptor – targeted agent interaction. In this application is shown a precise lc/ms method for analysis of drugs agents finasteride, tamoxifen, used in cancer therapy, and beclomethasone, well known agent for asthma managment.

Hymecromone purity (APK #386)

Hymecromone is a drug used as a choleretic and antispasmodic medicine to treat bile.

I

Ibuprofen (APK #307)

Ibuprofen is a substance from a group of non-steroidal anti-inflammatory drugs. In order for the drug release to be targeted on the basis of pH change (gradual release for up to 30 days), binding to a polymeric carrier is used.

Ipidacrine (APK #303)

Ipidacrine is a drug inhibitor of acetylcholinesterase produced for the treatment of memory disorders caused by various diseases. This drug was first synthetized by the National Research Centre for Biologically Active Compounds (Russian Federation).

L

Lactoferrin in goat‘s milk (APK #405)

Lactoferrin (LT) is a glycoprotein, which is one of the group of transferrins. It is an iron-binding protein with a mass of 80 kDa. Lactoferrin has microbial properties against many microorganisms (bacteria, viruses and fungi, including parasites). It is one of the non-specific components of the immune system and has anticancer and anti-inflammatory properties.

Lactoferrin is widely represented in various animal fluids, mainly milk, saliva and tears. It is separated from bovine milk to produce this key ingredients of infant formulas. Lactoferrin is also used as a food supplement to support the natural immune system, gut microbiome and healthy skin.

Lactoferrin in milk (APK #400)

Lactoferrin (LT) is a glycoprotein, which is one of the group of transferrins. It is an iron-binding protein with a mass of 80 kDa. Lactoferrin has microbial properties against many microorganisms (bacteria, viruses and fungi, including parasites). It is one of the non-specific components of the immune system and has anticancer and anti-inflammatory properties.

Lactoferrin is widely represented in various animal fluids, mainly milk, saliva and tears. It is separated from bovine milk to produce this key ingredients of infant formulas. Lactoferrin is also used as a food supplement to support the natural immune system, gut microbiome and healthy skin.

Lutein in food supplements (APK #413)

Lutein and Zeaxanthin are carotenoids used in various dietary supplements. The task of this application was to develop a method that replaces the dichloromethane mobile phase with non-chlorinated solvents.

M

Melatonin (APK #551)

Melatonin is a natural compound produced by various organisms. In human body this hormone is secreted in the brain, playing a crucial role in regulating the sleep-wake cycle. In addition to its endogenous functions, it is administered as a medication in the treatment of sleep disorders, such as insomnia and various circadian rhythm sleep disorders. In this application two different ASTRA® C18-HE columns were tested according to Ph. Eur. Monograph method for melatonin. Both columns show the excellent peak symmetry in conditions of assay determination and related substances method.

Methionine Hydroxy Analog MHA (APK #332)

MHA® feed supplement – in full name DL-2-hydroxy-(4-methylthio) butanoic acid, is used for its antibacterial and antioxidant effects in the feed. MHA® is also an organic source of calcium.

Microplastic Analysis and Additive Screening using Thermal Desorption/ Pyrolysis (TDP) DART-MS

Microplastics are more often appearing as contaminants in important water sources including our oceans and drinking supply. These small particles are not a single type of contaminant but a wide variety consisting of flame retardants, plastic stabilizers, and colorants. The presented TDP-DART-HRMS method requires no sample preparation and allowed for the rapid and direct screening of plastics of known and unknown sources without the need of any chromatographic separation. Three types of plastics were evaluated: polyethylene terephthalate (PET), polypropylene (PP), and low-density polyethylene (LDPE) from various sources. Additionally, an unknown plastic was analyzed. The unique combination of thermal desorption, direct ionization, high-resolution mass spectrometry and sophisticated postprocessing software for statistics and compound identification resulted in a improved detection and characterization of these concerning contaminants. The resulting high-resolution MS and MS/MS data was quickly processed and used to match against existing libraries with successful identification of the components that were present in the unknown sample.

Monitoring of Ethylene Oxide (APK #426)

Ethylene oxide monitoring system for machines and working environments enables to measure 1, 4, 6, 8 or 10 samples from various sample points according to user requirements (in economy one-channel version). The two-channel version gives double count of sample points with the same measurement interval. The system is composed of gas chromatograph (GC) with flame-ionization detector (FID), sample selector and gas box for up to 10 sample points, PC with ETOx control software and chromatography software (Clarity) and cabinet with hardware control modules. Whole system including control software is delivered as a turn key solution according to customer requirements.

Monosaccharides, disaccharides and sugar alcohol (APK #354)

The analysis of saccharides and sugar alcohol is one of the most common criteria in food and beverage analyses in the QC departments of manufacturers and the monitoring of authorities. This analysis of sugars and sugar alcohols is also used to detect food and beverage adulteration.

O

Oligonucleotides (APK #308)

The application shows the purification of nucleotides on ARION® column compared with our competitors‘
BDS and BDS-clone columns.

Oligonucleotides II (APK #348)

ARION® Polar C18 shows much better retention and resolution in GC 52% oligonucleotides analysis than competitive column developed for this particulate application.

Opioids and Tramadol and their metabolites by LC/MS (APK #349)

This application shows the LC/MS method for the most common opiates and their metabolites analyzed by toxicological labs.

Opioids APIs (APK #475)

Opioids can be used in medicine to treat pain and to manage withdrawal symptoms. ASTRA® Phenyl-Hexyl-HE column allows to separate the mixture of opioids with sufficient resolution coefficients in comparison to competitive’s column.

Organic acids (APK #355)

The identification and quantitative analysis of major organic acids in fruits and vegetables is considered very important for the food and beverage industry. Organic acids play a significant role thanks to their influence on flavour, stability and keeping quality. Organic acids are generated during the aerobic oxidation of carbohydrates, proteins and fats in most biological systems.

Organic acids (APK #439)

This application shows the determination of tartaric, malic, shikimic, lactic, citric and succinic acids. The quantity balance of these compounds influences on taste and flavor of wines and fruit juices. The ASTRA® column provides the simultaneous determination of organic acids with good peaks resolution at low limits of the concentrations.

P

Penicillin impurities (APK #314)

Penicillin is well know antibiotic found by Alexander Fleming. He isolated it from the mold Penicillium notatum. The application shows better separation of impurities in pharmaceutical production.

Phenoxymethylpenicillin impurities (APK #330)

This application shows Phenoxymethylpenicillin potasium impurities analysis in accordance with Ph. Eur. monograph.

Photosynthetic pigments (APK #483)

This application shows the method of analysis of the main photosynthetics pigments in extracts of Hibiscus leaves and extract from cells of the algae Emiliania huxleyi. ASTRA® C18-HE column allows to achieve good separation of the pigments, which is always challenging.

Polar pesticides by LC-MS/MS

Polar pesticides are commonly used in agriculture. This application shows the LC-MS/MS method for simultaneous determination of highly polar pesticides paraquat, diquat, chlormequat and mepiquat.

Potassium trichloroammineplatinate (II) (APK #385)

Potassium trichloroammineplatinate(II) (KTCAP) is key intermediate in the synthesis of novel platinum anticancer drugs.

Prepurification of adipokinetic hormones (APK #472)

Adipokinetic hormones (AKHs) are insect anti-stress hormones that maintain the biochemical and physiological homeostasis of the insect body (Kodrík, 2008). AKHs are octa, nona- or decapeptides with both termini blocked: the N-terminus by a pyroglutamate residue and the C-terminus by an amide. Typically, a specific antibody and ELISA method are used for their quantification in the insect central nervous system and haemolymph. For the latter, prior to executing the ELISA test, several pre-purification steps are required; they also involve a solid phase extraction cartridge.
In the test, the AKH from the firebug Pyrrhocoris apterus known as Pyrap-AKH was employed. Its structure is: pGlu-Leu-Asn-Phe-Thr-Pro-Asn-Trp-NH2 (Kodrík et al., 2000).

Preservatives in caffeinated beverages (APK #390)

Sodium and potassium salts of benzoic acid and sorbic acid are well-known food preservatives. The permitted amount in food is strictly regulated with the level depending on the food group. As an example, European regulation EC 1333/2008 sets the rules on food additives: definitions, conditions of use, labelling and procedures.

Preservatives in syrup (APK #356)

Sodium and potassium salts of benzoic acid and sorbic acid are well-known food preservatives. The permitted amount in food is strictly regulated with the level depending on the food group. As an example, European regulation EC 1333/2008 sets the rules on food additives: definitions, conditions of use, labelling and procedures.

Production of adhesives (APK #511)

The technological process of adhesives production, used in chipboards and OSB boards, consists of polymerization or condensation of solution of formaldehyde and technical urea. This process is controlled by continuously measurement of pH and temperature in the reactors in the so-called pH circuits. For these purposes, the FermPro process sensor type are used, their internal pressure well isolates the diaphragm from the measured medium and thus the electrode body does not stick. Single-channel transmitter to convert the signal for visualization is used.

Production of hair care products (APK #512)

Human hair are very sensitive to the pH variations of applied products. Apart the safety reasons, the pH range also has a big impact on performance of rinse-off cosmetic products. The control of pH is obligatory test in shampoos production. For these purposes, the CHS ChemCleen Lab® pH electrode is used. It is operating in wide range of temperatures and pH.

Production of printing machines (APK #420)

The printing machines use 4+4 ports with ethanol based paint solutions. Each channel has to be controlled for viscosity and for some customers, it has to be equipped with pH measurement. Some Print houses use these machines in explosive areas. In such cases, these instruments have to be delivered with ATEX approved sensors and transmitters.

Prostaglandins (APK #337)

Prostaglandin Active Pharmaceutical Ingredients (APIs) play a significant role in the bodies of humans and animals, and therefore are synthetised for use in ophthalmology, veterinary, and vascular treatment.

Purification of vitellogenin from bee haemolymph (APK #508)

Vitellogenins, glycolipophosphoproteins mostly known and well-characterized as precursors of yolk proteins, are involved in reproduction in the majority of oviparous animals. In insects, vitellogenins are typically synthesized in the fat body, from where they are transported via the haemolymph into growing oocytes. The honey bees are no exception: vitellogenin (mw about 200 kDa) is known to play crucial roles in bee reproduction, but its involvement appears to be multifunctional, since vitellogenin influences oxidative stress, nutrition, immunity, and honey bee longevity.

Pyrethroid insecticides (APK #517)

Flumetrin and fluvalinate are pyrethroid insecticides used to control mites in honeybee colonies. The treatment by these insecticides should be strictly controlled due to its toxic effects on health of honeybees. The described method
allows to determine flumetrin and fluvalinate in honey and related biological samples matrixes.

Pyrrolizidine alkaloids in herbal tea (APK #350)

Pyrrolizidine alkaloids belong to a class of plant toxins, which are associated with diseases in human and animal bodies.

Q

Quantitation of PFAS in Water by LC-HR-QTOF MS/MS

Per- and polyfluorinated alkyl substances (PFAS) are a diverse group of extensively used industrial chemicals. They are classified as persistent pollutants, with known environmental accumulation and global circulation. Due to their suspected toxicity, they are a growing threat to both humans and wildlife, and thus their analysis is an increasingly important task for analytical chemists. In this application a method of quantification of PFAS in water using a time-of-flight (QTOF) mass spectrometer is presented. In addition to the rapid, automated, and confident detection of expected and targeted PFAS compounds, analysis via this high resolution QTOF system offers the unique potential to detect unexpected or novel PFAS via retrospective reviewing of data sets. An unlimited number of compounds may be targeted in each run, and processing methods may be easily extended to include new PFAS compounds, providing flexibility for current and future regulatory criteria.

Quinolones (APK #410)

A Quinolone antibacterials are drugs used in veterinary medicine to treat bacterial infections. The analysis of residual amounts of drugs in food is important for monitoring the quality control of products of animal origin. This application clearly shows the separation of 16 quinolones using the LC/MS technique.

R

Rapid, Chromatography-Free Quantitative Workflow of the Potent Sedative Xylazine by DART-MS Analysis

Xylazine is a synthetic compound that has escalated concern within United States health and law enforcement agencies due to its increased use as an adulterant in the illicit street drug fentanyl. Numbers of opioid overdoses continue to be significant and the detection of xylazine as an adulterant in illicit drugs is increasing each year. Thus, interest to be able to measure this drug rapidly and effectively in mixed lab samples is escalating. A technique utilizing a chromatography-free workflow by Direct Analysis in Real Time ionization coupled with mass spectrometry (DART-MS) offers simple, quick identification of xylazine. For this study, a mixture containing xylazine, fentanyl, and chlorpromazine spiked into drug-free urine was used to demonstrate the effectiveness of DART-MS. The linear responses of the drugs with respect to the QC samples and across a large detection range ensure that this chromatography-free workflow will be able to detect samples at referenced levels. The ability to conduct rapid analysis of such drugs allows for quicker and more confident treatment decisions to overdose victims, who may not fully respond to current overdose treatments.

Related substances of cefazolin sodium (APK #516)

Separation of impurities with close structure is always challenging in pharmaceutical industry due to the strict requirements to acceptance criteria of chromatographic method and low limits for impurities quantification. This application shows the method for determination of related substances of cefazolin sodium by the Ph. Eur. Monograph. ARION® Polar C18 column allows to separate all impurities according to the method.

Residual Solvents (APK #419)

Residual solvents control is a mandatory test for active pharmaceutical ingredients. Column LION™ LN-1 provides fast, robust and reproducible separation for most commonly used solvents.

S

Selected C18 phases comparison (APK #396)

This application shows a selectivity comparison of different C18 phases on QC mixture. It compares the hydrophobicity parameter of ARION® and ASTRA® HPLC columns with selected columns from other manufacturers.

Steroids (APK #488)

A quantitative determination of steroids is a valuable tool in a clinical practice in diagnostic of the steroid-related diseases. This method shows the simultanious analysis of wide panel of steroids by sensitive LC-MS/MS method.

Stevia glycosides (APK #387)

Stevia rebaudiana Bertoni is a plant used for its sweet taste. It includes glycosides, which are used as sweeteners in the food industry.

Sugars and Organic acids in Balsamic vinegar (APK #561)

Balsamic vinegar is celebrated for its rich flavor, which is the result of a delicate balance between sugars and organic acids. Analyzing these components is essential for producers aiming to maintain quality and create distinct flavor profiles. Sugars, primarily glucose and fructose, contribute sweetness, while organic acids, notably acetic acid, provide the characteristic tang. Understanding the levels of
these compounds helps ensure consistency across batches, enhances the overall taste, and allows for adjustments that create a harmonious balance. Additionally, rigorous analysis supports quality control and regulatory compliance. Many regions impose strict standards on balsamic vinegar production, and regular testing ensures adherence to these guidelines, fostering consumer confidence in the product.
In summary, the analysis of sugars and organic acids in balsamic vinegar is crucial for crafting high-quality products that delight consumers and uphold authenticity in this beloved condiment.

Sugars control in tomatoes and ketchups (APK #555)

A taste of tomato products depends on content of the main food sugars, such as sucrose, glucose and fructose. Sugars are analysed continuously during the products manufacturing at the different steps of a process. The content of sugars in tomatoes is highly dependent on the geographical origin, ripeness and transportation conditions. This application highlights the analysis of food sugars in several kinds of tomatoes and ketchups using ASTRA® Sugar Ca column.

Sugars in alcoholic beverages (APK #554)

Glucose and fructose are the major fermentable sugars presented in alcoholic beverages. Accurate determination of sugars in alcoholic fermentation process is a key point in the winemaking. Column ASTRA® Sugar Ca provides a perfect estimation of variable amounts of sugars in fermented alcoholic beverages.

Sugars in honey (APK #552)

Honey has been used since ancient times as a food and in medicine. Its composition is highly variable depending on its botanical and geographical origin. It is mainly composed of a mixture of different sugars, which daily intake should be controlled due to the nutritional reasons. In this application the chromatographic profiles of sugars in several honey varieties are shown.

Sugars in non-alcoholic beverages (APK #553)

Non-alcoholic beverages are popular among children and adolescents. Due to high content, the sugars separation and quantification in these products are often challenging. In the tested non-alcoholic beverages, a several sugars, such as sucrose, glucose and fructose, were detected in a different proportions.

Sweeteners and organic acids (APK#313)

This application shows the separation of three groups of compounds in parallel: non-nutritive sweeteners, organic acids and xanthine derivatives.

Synthetic Opioid in Cleaning Validation (APK #375)

This API is a synthetic opiod with around 100 times stronger effect than morphine. This application shows API monitoring during a validation of a cleaning process at trace levels.

Synthetic prostaglandin (APK #438)

Synthetic analogue of prostaglandin F2α (PGF 2α) is used in veterinary medicine as luteolytic agent for the induction of estrus and the treatment of reproductive disorders in cattle, swine, and horses. This application shows the determination of synthetic prostaglandin according to proprietary method. ASTRA® C18-HE column provide perfect peak symmetry in a long sequence of analysis.

T

Tamsulosin hydrochloride analysis (APK #292)

Tamsulosin hydrochloride is used to treat the symptoms of an enlarged prostate. This drug is an alpha-blocker that works by relaxing the muscles in the prostate and the bladder. Tamsulosin is sold under various trade names, e.g. Flomax, Urimax, Contiflo XL, Mesir LP, Prostanil MR, Tamsin, Fokusin, etc. The application note shows the chromatogram of the determination of the tamsulosin hydrochloride content according to the proprietary method.

TargetScreener 4D workflow: Providing solutions in environmental monitoring studies

Thousands of chemicals originating from anthropogenic sources are released daily into the environment. Therefore a comprehensive and systematic environmental monitoring is necessary for the establishment of effective mitigation measures for contaminants with persistent bioaccumulative and toxic characteristics (PBT). In this study, a CCS-aware database was etablished, containing LC-ESI-TIMS-HRMS data of more than 1,000 environmental contaminants, typically found in environmental matrices. The included compounds belonged to several classes, such as pharmaceuticals, personal care products, drugs of abuse, pesticides, as well as their transformation products. As test samples, different environmental matrices were utilized, representing the challenge and needed performance for a comprehensive screening approach. Trapped Ion Mobility Spectrometry (TIMS) coupled to High Resolution Mass Spectrometry (HRMS) were used to enhance the reliable identification of contaminants.

Thiopental impurities (APK #411)

Thiopental sodium is a medicine given intravenously to induce short duration general anaesthesia. The content of active pharmaceutical ingredients and related substances should be strictly controlled for injected drugs. This application shows a method for the simultanious determination of thiopental and related substances. ASTRA® column allows all impurities to separate according to the method and provides an accurate and robust determination of thiopental.

Total Petroleum Hydrocarbons (APK #389)

Total petroleum hydrocarbons index (TPH) is a typical environmental analysis. It has replaced the infrared spectroscopy method using problematic solvents, i.e. Freons. This gas chromatography analysis (GC) monitors hydrocarbons between n-decane and n-tetracontane. These two hydrocarbons are used as the range marker and injection efficiency control. Aditionally, the GC method has an important advantage – this can show a type of hadrocarbon contamination (e.g. gasoline, naphtha, motor oil) and weathering status (some n-alkanes disappear during their stay in the enviroment).

Tramadol by LC/MS (APK #364)

Tramadol is a centrally acting opioid analgesic belonging to the pharmacotherapeutic group of opioid analgesic. Tramadol is sometimes used in combination with Paracetamol to treat moderate to severe pain suffered by patients with various types of pain. This application has been developed to monitor right drug dosing.

Triazoles (APK #414)

Triazoles are used as intermediates for API production. This application shows the separation of all triazoles in one analysis.

Tween 80 in pharmaceuticals (APK #521)

Tween 80 is used to stabilize aqueous solutions with drugs and also as an emulsifier in the pharma industry. It is also used as an additive in vaccine production. Currently it is added to Covid-19 vaccines. Column CHROMSHELL® C18-XB provides a good tailing factor for Tween 80 and excellent separation from others components in comparison to competitor’s column.

U

Ultra-fast chromatography-free analysis of carbohydrates for food

A quantitative method was developed for the direct infusion of samples for the analysis of di-, tri- and malto-saccharides (length of 4–10 monomers) that could be accomplished in one minute. A rapid and a simple method for the analysis of sugar which is applicable across diverse types of food matrices could be useful to the food industry in tackling the large number of the analysis required in various applications including nutritional labeling and also in reducing the extensive time currently required to complete this analysis.

V

Vitamin D in dry blood spot (APK #325)

Vitamin D is a group of steroids that have various effects on the human body and support the immune system. This application shows LC/MS/MS separation of the hydroxylated and non-hydroxylated forms of vitamin D2 and vitamin D3. The amount of their hydroxylated forms determines the total vitamin D in a blood sample. Non-hydroxylated forms of vitamin D2 and vitamin D3 are important for food analysis. The LC/MS/MS chromatogram shows the separation of all the above mentioned analytes.

Vitamin E and provitamin A in food supplements (APK #392)

Beta-carotene is one of the group of carotenoids. It is a precursor of Vitamin A and plays a significant role as an antioxidant. Alpha-tocoferol is a type of Vitamin E. This applications shows the separation of both compounds in a sample of a food supplement.

Vitamins A and E (APK #383)

Fat-soluble vitamins are monitored not only in patient’s samples, but are also the subject of quality control in various food and dietary supplements.

Vitamins B (APK #384)

The group of water-soluble B vitamins is widely monitored in dietary supplements and in food. They play an important role in cell metabolism and the synthesis of red blood cells. This application shows the HPLC separation of B1, B2, B3, B6 and B12 vitamins.

Voriconazole and Posaconazole in blood plasma (APK #347)

Voriconazole and Posaconazole are antifungal drugs that are monitored in plasma and urine. Spiked samples based on a blood plasma from blood donors at transfusion station were used in this application note.

W

Wheat pigments (APK #344)

This application has been developed by ALGATECH, Institute of Microbiology of the Academy of Sciences, Czech Republic. Chlorophylls and carotenoids are essential cofactors for the oxygenic photosynthesis. As the content and stoichiometries of individual pigments are significantly varying in plant leaves under different environmental conditions, the quantification of pigments is important for the understanding of plant physiology, but also for the food-quality monitoring.

Z

Zopiclone impurities (APK #540)

Related substances control is a mandatory test in routine specification of active pharmaceutical ingredient. Сhromatographic method should meets the strict system suitability requirements that is often challenging due to close retention times of the studied compounds. ARION® column shows a robust and precise results in control of Zopiclone related substances in a long sequense of analysis.

С

Сhemicals of emerging concern in river water (APK #469)

The chemical pollution cause huge damage to the aquatic environment.The simultaneous determination of pollutants with different physico-chemical properties, such as pesticides, metabolites, personal care products, plastic-related chemicals is always challenging. The application shows a sensitive LC/MS method for fast determination of diverse group of pollutants in river water.