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Choice of column depending on the injection size and its capacity
| ID (mm) | Injection value (µl) | Column Capacity (mg) | Flow rate (ml/min) |
| 4,6 | 5 - 100 | 1 | 0,5 – 2,0 |
| 10 | 100 - 1000 | 5 | 4,0 – 15,0 |
| 21,2 | 1000 - 5000 | 20 | 10 – 50 |
| 30 | 2000 – 10 000 | 40 | 40 – 100 |
| 50 | 5000 – 20 000 | 120 | 100 – 300 |
| 100 | 10 000 – 50 000 | 500 | 400 - 1000 |