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The linear velocity is an important parameter in chromatography. It has an influence on chromatography resolution and therefore setting the dead volume is basic part of method development.
To set a dead time, inject 2 µl of a non-retained gaseous substance, which is compatible with the detector. Take a gas-tight syringe and draw the headspace over neat compound. Accurately mark the injection starting time and peak elution time.
Detector | Compound |
---|---|
FID | methane, propane, butane |
ECD | methylen chloride, air (at lower oven temperatures) |
TCD | methane, butane, air (at lower oven temperatures) |
NPD | acetonitrile, air (at lower oven temperatures) |
MS | propane, butane, argon, air (at lower oven temperatures) |
PID | acetylene, ethylene |
Note: Some compounds may be slightly retained on thick-film phase, however, they will be reproducible for similar column types.