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This technical note shows possible types of connection between column and LC system. Bad connection may influence peak separation and should be avoided. The right connection is presented below.
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Many GC and LC problems can be avoided with routine preventive maintenance. If you are seeking the cause of the chromatography problem, go step by step. Never make more changes in your instrument at the same time, otherwise you will never find, what caused the problem.
Select your category of chromatography to read more about the troubleshooting: