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  1. Chromservis
  2. Chromatography
  3. MetAmino kit
  4. MetAmino description
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Chromatography

Flash and Preparative HPLCGC/MS-TOF
    GC/MS/MS
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        MetAmino description

        Introduction

        Amino acids are key building blocks of life and play pivotal role in various metabolic pathways. They mostly act as intermediates often not directly involving proteins. Due to their chemical complexity and dynamic range, their reliable quantitative and qualitative analysis in biological fluids and tissues is crucial for nutritional information, compound identification and diagnostics.

        For that purpose, a simple, elegant and thus far the most expeditious method for an invaluable amino acid analysis has been developed. LC/GC-MS based Metamino® kit offers comprehensive solution up to 75 metabolites including basic proteinogenic amino acids, biogenic amines and coenzymes with the possibility to a further analyte extension.

        Description

        Our LC-MS/GC-MS MetAmino® kits provide fast, robust, reproducible and accurate procedure for the amino acid quantification, where both sample handling and chromatographic separation are taken into account.

        Using liquid chromatography (LC) coupled to mass spectroscopy (MS), the method is based on micro-solid phase extraction (MSPE) using special MetAmino® spin filter with proprietary media. MetAmino® microspin filter absorbs derivatized amino acid analytes, which are eluted through the integrated 0.22 μm membrane and afterwards injected into a MetAmino® HPLC column, undergoing a LC-MS analysis. In total, sample preparation takes around 8 minutes and sample analysis 12 minutes, thus the entire experimental time is only 20 minutes.

        While using gas chromatography (GC) coupled to MS, the sample is firstly derivatized followed by liquid-liquid microextraction step (LLME). Derivatized analytes migrate into the organic layer which is then directly amenable for a quick detection on GC-MS system. In GC-MS kit, sample preparation time has been shortened to 5 minutes and sample analysis runs 12 minutes. Total experimental time is roughly 17 minutes.

        This unique kit meets all emergency requirements and needs of high throughput laboratories and is designed for all end-users seeking high separation efficiency.