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  1. Chromservis
  2. Chromatography
  3. Unit conversions
  4. Unit conversions in chromatography
  5. Estimation of pressure on HPLC columns depending on particle size, diameter and column length
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Chromatography

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        Estimation of pressure on HPLC columns depending on particle size, diameter and column length

        Particle size (µm) ID (mm) Flow (ml/min)

        Pressure 150mm* (PSI)

        Pressure 250mm* (PSI)
        3 4.6 0.5 – 1.0 1000 1500
        5 3.0 0.4 – 0.8 750 1250
        5 4.6 1.0 - 2.0 700 1100
        5 10.0 5.0 - 10.0 750 1250
        10 4.6 2.0 - 5.0 400 600
        10 10.0 10.0 - 20.0 500 800
        10 21.2 20.0 - 40.0 300 500

        * When the flow rate is at the lower limits of the recommended flow rates