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  1. Chromservis
  2. Sample preparation
  3. Filtration
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Sample preparation

IntroductionFiltrationAir sampling pumps and accessories

Filtration

SKC Filter Selection Guide

Material Abbr. Common Pore Sizes Main Properties Air Sampling Applications
Mixed Cellulose Ester MCE or MEC 0.45 µm
0.8 µm
1.2 µm
5.0 µm 		Hydrophilic
Readily soluble for atomic absorption analysis
Readily rendered transparent for transmitted light microscopy
Dissolve and clear easily		 Metal dust analysis
Asbestos and man-made fibers
Polyvinyl Chloride
(Pure Homopolymer)
GLA-5000		 PVC or PH-PVC 0.5 µm
0.8 µm
5.0 µm 		Hydrophobic
Non-oxidizing surface
Silica-free
Low ash
Low tare weight for gravimetric analysis		 Gravimetric analysis
Hexavalent chromium
Polytetrafluoroethylene PTFE 0.5 µm
1.0 µm
2.0 µm
5.0 µm 		Hydrophobic
Inert to solvents, acids, and bases
Autoclavable		 Alkaline dusts
Polynuclear aromatics
Pesticides
Isocyanates
Polycarbonate PC 0.3 µm
0.4 µm
0.8 µm 		Hydrophobic
Microscopically smooth surface
Straight-through pores
Extremely thin (10 to 20 µm) and transparent
Autoclavable		 Scanning electron microscopy
Asbestos fibers
Silver AG 0.45 µm
0.8 µm 		Wide solvent compatibility
Higher temperature tolerance
Autoclavable
Uniform porosity and thickness		 Bromine
Asbestos by TEM
Silica by x-ray diffraction
Glass Fiber GF or FG Liquid Nominal Rating 1.0 µm Partially hydrophobic
Higher temperature tolerance
Autoclavable
High particulate retention		 Pesticides
Ethylene glycol
Coarse gravimetric
Isocyanates
Quartz QZ Liquid Nominal Rating 1.2 µm Same as fiber glass
Autoclavable
Low level metals content
High temperature 300 C		 Gases
Aerosols
PM10
Diesel particulates
Cellulose CEL Nominal Autoclavable
Uniform strength
Ashless (Type 40)		 AA
HPLC

Air Monitoring Cassette Blanks

Air Monitoring Cassette Blanks
Diameter (mm) Type Material/Properties Qty. Cat. No.
25 2-piece Styrene, clear 50 225-2-25LF
100 225-2258
25 3-piece Styrene, clear 50 225-3-25LF
100 225-2259
25 Middle ring Styrene, clear, 1/2 inch height 50 225-304
For use with SKC Omega brand cassettes only
37 2-piece Styrene, clear 10 225-2LF
50 225-2050LF
250 225-2250
37 3-piece Styrene, clear 10 225-3LF
50 225-3050LF
250 225-3250
37 2-piece Styrene, opaque brown 50 225-4
37 3-piece Styrene, opaque brown 50 225-8451
25 Middle ring Styrene, clear, 1/2 inch height 50 225-300R
For use with SKC Omega brand cassettes only
Solvent-resistant* Air Monitoring Cassette Blanks
Compatible with most solvents*
Diameter (mm) Type Material/Properties Qty. Cat. No.
25 3-piece, goblet style Polypropylene, opaque white 50 225-8585
37 2-piece Polypropylene, opaque white 50 225-8483
37 3-piece Polypropylene, opaque white 10 225-45
37 3-piece Polypropylene, opaque white 50 225-45A
Conductive Air Monitoring Cassette Blanks
For reducing sample loss due to electrostatic effects; required for asbestos sampling
Diameter (mm) Type Material/Properties Qty. Cat. No.
25 3-piece, 2-inch middle cowl Polypropylene, conductive black 50 225-3-23
25 2-piece Polypropylene, conductive black 50 225-2257
25 3-piece 1/2-inch middle ring Polypropylene, conductive black, with ½-inch spacer ring (rather than 2-inch cowl) 50 225-329
37 3-piece Polypropylene, conductive black 50 225-309
37 2-piece Polypropylene, conductive black 50 225-308
47 2-piece Polypropylene, conductive black 16 225-8496
47 3-piece Polypropylene, conductive black 16 225-8497

CA Filters

Cellulose Acetate(CA)CA Filters

  • Composition: Mixture of triacetate and diacetate
  • Characteristics:Low static charge and high strhgth
  • Sterilizable: May be repeatedly sterilized without loss of integrity or change in bubble point
  • Clean: 0.1 wt% aqueous extractables
  • Related to MCE membranes: - improved solvend resistance to low molecular weight alcohols - better heat resistance - lower protein binding
    Specifications for Cellulose Acetate (CA)
    ore Size [µm Bubble Point [MPa] Flow rate (Water)[ml/min/cm2] Flow rate (Air) [L/min/cm2] Porosity [%] Thickness [µm]
    0.20 ≥0.25 16 2 66 125
    0.45 ≥0.17 35 4 68 125
    0.80 ≥0.068 160 14 72 125
    3.00 ≥0.034 500 54 78 135

Specifications for CE
Pore Size [µm] Color Surface Bubble Point [MPa] Flow rate (Water)[ml/min/cm2] Flow rate (Air) [L/min/cm2] Porosity [%] Thickness [µm]
0.10 White Plain ≥0.24 2.7 0.67 65 110
0.20 White Plain ≥0.37 17.5 2.4 73 133
0.30 White Plain ≥0.28 30 3.7 75 114
0.45 White Plain ≥0.24 45 5.0 78 145
0.45 White Grid ≥0.16 80 8.0 79 142
0.65 White Plain ≥0.14 120 11.2 79 150
0.80 White Plain ≥0.11 165 15.0 80 150
1.00 White Plain ≥0.096 220 20.4 80 150
3.00 White Plain ≥0.070 300 28.3 81 155
5.00 White Plain ≥0.058 400 40.9 81 160
0.45 Black Grid ≥0.22 50 5.0 78 135
0.80 Black Grid ≥0.10 170 15.0 80 145
0.45 Green Grid ≥0.22 50 5.0 78 135
0.80 Green Grid ≥0.10 170 15.0 80 145

Ash Content of White Plain MCE Membrane Filters (ppm)
Al <0.2 K 6.0 Ni <5.0
Ca 140.0 Li <1.0 Pb <1.0
Cd <0.5 Mg 10.0 Si <20.0
Cr 8.0 Mn <0.5 Sn <5.0
Cu <1.0 Mo <1.0 Ti <1.0
Fe <5.0 Na 10.0 Zn <1.0

Filtration

FiltrationFilter media selection guide

there are many filtration materials used in filtration process. Filtration is mostly used in various applications, like membrane filtration of water or organic samples, syringe filters in HPLC or air sampling of dust aerosol. Table listed belwo shows most commonly used filter media. by clickin on particulate filter material you will find detailed information.

Below listed materials are used for all that in following applications:

  • Pressure filtration
  • Vacuum filtration
  • Syringe filters (mostly in HPLC and UHPLC)
  • Asbestos sampling
  • Dust aerosol sampling
  • Chemical vapours sampling
  • Determination of dissolved compounds
Membrane filters
Type Description Max. temperature Refractive index
MCE Mixed Cellulose Esters 130°C 1.5
CA Cellulose Acetate 180°C 1.47
PTFE (Hydrophilic) PTFE - -
PTFE (Hydrophobic ) PTFE 120 - 160°C -
PC Polycarbonate 140°C -

MCE Filters

Mixed Cellulose Esters (MCE)MCE Filters

  • Composition: Mixed cellulose esters includes cellulose nitrate, also known as nitrocellulose, and cellulose acetate
  • High porosity: Provide superior flow rates
  • High protein binding: Can be blocked by pretreatment or utilized in applications
  • High purity: Triton-free and non reactive to pyrogens
  • Autoclavable: Withstand autoclaving temperatures up to 130 C without adversely affecting bubble point rate or microbiological recovery
  • Rapid wetting time: <3 seconds to wet a 47 diameter disc with aqueous 1% methylene blue

Specifications for MCE
Pore Size [µm] Color Surface Bubble Point [MPa] Flow rate (Water)[ml/min/cm2] Flow rate (Air) [L/min/cm2] Porosity [%] Thickness [µm]
0.10 White Plain ≥0.24 2.7 0.67 65 110
0.20 White Plain ≥0.37 17.5 2.4 73 133
0.30 White Plain ≥0.28 30 3.7 75 114
0.45 White Plain ≥0.24 45 5.0 78 145
0.45 White Grid ≥0.16 80 8.0 79 142
0.65 White Plain ≥0.14 120 11.2 79 150
0.80 White Plain ≥0.11 165 15.0 80 150
1.00 White Plain ≥0.096 220 20.4 80 150
3.00 White Plain ≥0.070 300 28.3 81 155
5.00 White Plain ≥0.058 400 40.9 81 160
0.45 Black Grid ≥0.22 50 5.0 78 135
0.80 Black Grid ≥0.10 170 15.0 80 145
0.45 Green Grid ≥0.22 50 5.0 78 135
0.80 Green Grid ≥0.10 170 15.0 80 145

Ash Content of White Plain MCE Membrane Filters (ppm)
Al <0.2 K 6.0 Ni <5.0
Ca 140.0 Li <1.0 Pb <1.0
Cd <0.5 Mg 10.0 Si <20.0
Cr 8.0 Mn <0.5 Sn <5.0
Cu <1.0 Mo <1.0 Ti <1.0
Fe <5.0 Na 10.0 Zn <1.0