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References

ReferencesTestimonials

MetAmino Kit testimonials

InventionBio S.A. Laboratory

In our laboratory, MetAmino Kit® was used to determine protein amino acids (such as glycine, serine, arginine, threonine, alanine, proline, valine, lysine, histidine, leucine, phenylalanine, methionine, etc.) in feed samples and lyophilisated intestinal content. Samples after acid hydrolysis were derivatized using MetAmino Kit and finally there were analyzed on LC-MS/MS. The developed method of sample preparation using MetAmino ensures repeatable, reliable analysis results and determination of feed digestibility and amino acid bioavailability.

We recommend using the MetAmino Kit for simple samples derivatization and reliable tool for the identification and quantification of amino acid. It is easy to use and it contributed to the successful results of our research projects.

Anna Filipiak-Szok, Ph.D.

P.J. Šafárik University, Faculty of Medicine

I am satisfied with the MetAmino Kit® because of its complexity. It is possible to quickly obtain necessary results. Each component for the final derivatization is enclosed and the sample derivatization is easy.

We have used it for monitoring of metabolites in STD1 and STD2 in plasma samples from COVID patients with a comparison to a healthy group. The experimental procedure is easily explained. I also appreciate that the column is enclosed in the kit.

RNDr. Soňa Tkáčiková, PhD.

Comenius University, Jessenius Faculty of Medicine

We had the opportunity to use the MetAmino Kit® for liquid chromatography (LC) in combination with mass spectrometry (MS) to determine polar substances in various biological matrices, such as culture media and blood serum or plasma. We can say without hesitation, that we were extremely satisfied with the obtained results. The kit provided us with all the necessary reagents, accessories and clear instructions to perform derivatization and analysis with high accuracy and sensitivity. One aspect that impressed us, was its simplicity of use. The instructions were clear and concise, which allowed us to quickly familiarize ourselves with the process.

The derivatization of substances itself is not so time-consuming and contributes to increasing the sensitivity of the determination of individual compounds. Thus, it reduces the limit of detection or quantification compared to other methods without derivatization. It is therefore possible to determine substances that are present in low concentrations. It is suitable for the analysis of substances that are difficult to quantify, using other methods, such as polyamines. At the same time, it is also possible to determine substances with a low molecular weight that can be degraded in the ion source. From our own experience we can mention glycine, alanine, and serine as problematic, which under normal circumstances can decarboxylate under the influence of high voltage, which we managed to prevent by using the MetAmino kit. Subsequently, we managed to successfully determine them in our samples. It is therefore a suitable complementary method to other methods that are limited in this respect. Another advantage is the short time of the LC-MS analysis itself and the simple evaluation of the obtained data.

Overall, we are more than satisfied with this liquid chromatography and mass spectrometry kit. It provides excellent results, is easy to use and has contributed to the successful execution of our research projects. We recommend it to all laboratories and researchers who need a reliable tool for the identification and quantification of molecules.

Mgr. Radovan Murín, Ph.D. & Mgr. Jakub Šofranko

J.E. Purkyně University

UJEP The MetAmino Kit® was used for the determination of amino acids in soils with a focus on the rhizosphere. The tested soil was contaminated with organic pollutants (diesel, PAH). If we compare the MetAmino Kit with the direct injection method without derivatization, higher and narrower peaks were achieved for the monitored amino acids. The advantage of the kit is the delivery of a complete set for sample preparation including isotopically labeled standards.

Sylvie Kříženecká, PhD.