YMC_Analysis-and-Purification-of-Oligonucleotides-by-AEX-and-IP-RP

This Whitepaper …
…is written for all chromatographers who deal with the analysis and / or purification of oligonucleotides. The focus is on anion exchange chromatography (AEX) and
ion pair reversed phase chromatography (IP-RP). For both modes, the main aspects are the selection of a suitable stationary and mobile phase as well as the optimum temperature.
Of course, the special features of the large-scale process are also taken into account.

In this whitepaper, you are guided through the following aspects:

• Introduction to Therapeutic Oligonucleotides by Valentina D’Atri, PhD, University of Geneva
• Separation Modes in Liquid Chromatography for Therapeutic Oligonucleotides
• Anion Exchange Chromatography
• Ion-Pairing Reversed Phase Chromatography
• Important Considerations for Loadability and Scale-Up

• YMC_Analysis-and-Purification-of-Oligonucleotides-by-AEX-and-IP-RP 10064 kB