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The LION® LN-FAME HT column with dimensions 60 m x 0,25 mm (0,20 µm d.f.) has been used in method development for the fatty acid profiling in plasma lipid classes in our Atherosclerosis Research Laboratory at First Faculty of Medicine, Charles University in Prague, Czechia. This column withstands high temperatures needed to elute cholesterol from the system when analysing cholesteryl ester profiles. Separation of peaks critical for the methyl ester analyses is also possible on this type of column.
RNDr. Marek Vecka, PhD.