ZearalaTest - immunoafinity columns, Fluorometer & HPLC (25/box)

Immunoffinity columns are used for isolation of Zearalenone from samples followed by HPLC or the use of fluorometr.Ze­aralenone is an estrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminization in animals at concentrations in feed of about 1 ppm. Higher concentrations (50–100 ppm) can interfere with conception, ovulation, implantation, fetal development, and the viability of newborn animals.

ZearalaTest is safe and simple. It can be performed in less than 15 minutes and requires no special skills. Results may be recorded using a digital fluorometer readout or automatic printing devices. ZearalaTest is also ideal as the cleanup step for any HPLC or TLC analysis.

Benefits

• Convenient – For use with fluorometer or HPLC
• Durable – Long shelf life; requires no refrigeration
• Versatile – For use with a variety of samples
• Quick – Less than 15 minutes to isolate toxin*
• Wide Range – Detects levels as low as 0.1 ppm and

as high as 5 ppm

• Safe – Requires less toxic materials than conventional methods

Here’s How Simple Zearalatest is to Use:

Extract Sample

• Grind and weigh sample
• Add salt and methanol/water or acetonitrile/water mixture
• Filter

Dilute and filter

• Dilute extract
• Filter

Absorb and Elute

• Pass filtrate over ZearalaTest affinity column
• Wash column
• Elute zearalenone and collect in cuvette

Measure

• Add Developer and place cuvette into a calibrated fluorometer, or
• Inject Eluate into HPLC
• Determine zearalenone concentration