Xtrackt Hydrophobic + Carboxylic H/F 500mg/6mL, 50 pkg

XtrackT® large particle bonded phases allow for uniform gravity flow for most blood and urine samples. A single column provides extraction for a broad spectrum of compounds with selective elution of acid, neutrals, steroids and bases. XtrackT® large particle (80–200 μm) silica gels are available with hydrophobic, hydrophilic, ion exchange or copolymeric phases, including DAU mixed mode. XtrackT® is recommended for viscous sample matrices or for gravity flow applications.

WCX is a mid-polarity sorbent and weak cation exchanger. It can be used with a wider range of counter-ions than lower pKa sorbents like SCX, and will demonstrate easier elution of quaternary amine functionalized analytes.

General method for column with 100mg of sorbent

Condition

1 mL Methanol

Equilibrate

1 mL Water pH 6–7

Load

Pretreated sample at pH 6–7

Wash 1

1 mL Water pH 6–7

Wash 2

1 mL Methanol, dry under vacuum for 2–5 min

Elute any base

1 mL 5% Formic acid in Methanol

Elute weak bases

1 mL 5% NH4OH in Methanol

Recommended applications:

Strong cations