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T-2/HT-2 - Immunoafinity HPLC Columns (25/box)

T-2/HT-2 toxins are produced by various Fusarium fungi that naturally occur in wheat and other cereal grains.

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Product description

Product description

T-2/HT-2 toxins are produced by various Fusarium fungi that naturally occur in wheat and other cereal grains. Ten times more toxic than the potentially lethal contaminant deoxynivalenol (DON),T-2/HT-2 toxins attack the immune system in livestock, causing serious health problems ranging from skin lesions and weight loss to shock and hemorrhagic fever. Researchers have also linked T-2/HT-2 to the devastating human disease alimentary toxic aleukia (ATA). The mounting evidence on the deadly effects of T-2/HT-2 exposure has raised concerns among food safety regulators worldwide and underscores the importance of test methods that can detect trace amounts of these toxins before they reach human and animal consumers.

VICAM’s new T-2/HT-2™ HPLC test kit combines proven immunoassay technology with high-performance liquid chromatography (HPLC ), the preferred analytic method of laboratories around the world, to detect and measure T-2/HT-2 toxins. The highly selective monoclonal antibodies in VICAM patented immunoaffinity columns streamline sample cleanup and, when used in conjunction with UV detection, provide precise, reliable readings of contamination levels ranging 1,000 ng to 100 ng.* This sensitive, reliable HPLC-only test has a long shelf life and can be used with a variety of samples. No other test method comes close to VICAM’s for speed, precision, and specificity.

Benefits

  • Powerful: Designed exclusively for HPLC use
  • Convenient: Uses same methods as other VICAM tests
  • Durable: Long shelf life; requires no refrigeration
  • Versatile: Can be used with a variety of samples
  • Wide Range: Reliable readings of contamination levels ranging 1,000 ng to 100 ng.*
  • Safe: Requires less toxic materials than other methods

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Parameters

Parameters

Brand:
Category: Laboratory instruments and accessories / Chromatography / IAC columns

Lower limits may be achieved with other methods of detection//

Here’s How Simple T-2test HPLC is to Use:

Extract Sample

  • Grind and weigh sample
  • Add salt and methanol/water mixture
  • Blend and filter

Dilute and Filter

  • Dilute extract
  • Filter

Absorb and Elute

  • Pass filtrate over T-2/HT-2 HPLC affinity column
  • Wash column
  • Elute T-2/HT-2 and collect in cuvette

Measure

  • Dry and reconstitute eluate
  • Inject into HPLC
  • Determine T-2/HT-2 concentratio
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