FumoniTest WB - Immunoafinity HPLC Columns (25/box)

Immunoffinity columns are used for isolation of Fumonisins from samples followed by HPLC. Fumonisins are mycotoxins produced by the fungus Fusarium moniliforme. Frequent, almost universal inhabitants of corn, Fumonisin B1 and fumonisin B2 are present in most corn samples tested. Fumonisin is thought to cause equine leukoencephalo­malacia in horses, swine pulmonary edema, and human esophageal cancer.

Characteristics

• For use with HPLC
• Isolation of fumonisins B1, B2, and B3
• Durable – Has a long shelf life
• Versatile – For use with a variety of samples
• Quick
• Wide Range
• Total Volume: 3 ml

Here’s How Simple Fumonitest WB is to Use:

Extract Sample

• Grind and weigh sample
• Blend with extraction solvent
• Centrifuge
• Filter

Dilute and Filter

• Dilute extract
• Filter

Absorb and Elute

• Pass filtrate over FumoniTest WB affinity column
• Wash column
• Elute fumonisins and collect in a cuvette
• Dry eluate and reconstitute

Measure

• Add developers; inject eluate into HPLC
• Determine fumonisin concentration