FumoniTest - Immunoafinity columns for Fluorometer & HPLC (25/box)

Immunoffinity columns are used for isolation of Fumonisins from samples followed by HPLC or the use of fluorometr. Fumonisins are mycotoxins produced by the fungus Fusarium moniliforme. Frequent, almost universal inhabitants of corn, Fumonisin B1 and fumonisin B2 are present in most corn samples tested. Fumonisin is thought to cause equine leukoencephalo­malacia in horses, swine pulmonary edema, and human esophageal cancer.

Characteristics

• Convenient: For use with fluorometer or HPLC
• Comprehensive: Tests for fumonisins B1, B2, and B3
• Durable: Has a long shelf life
• Versatile: For use with a variety of samples
• Quick
• Wide Range
• Total Volume: 1 ml

Here’s How Simple FumoniTest is to Use:

Extract Sample

• Grind and weigh sample
• Add salt and methanol/water mixture
• Filter

Dilute and Filter

• Dilute extract
• Filter

Absorb and Elute

• Pass filtrate over FumoniTest

affinity column

• Wash column with buffers
• Elute fumonisins and collect

in cuvette

Measure

• Add developers and place cuvette into a calibrated fluorometer, or
• Inject eluate into HPLC
• Determine fumonisin concentration