Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed. DON is often present with other mycotoxins and has been isolated from grains and feeds throughout the world at levels as high as 92 ppm.
Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed. DON is often present with other mycotoxins and has been isolated from grains and feeds throughout the world at levels as high as 92 ppm. DON has been implicated in moldy corn toxicosis of swine and may play a role in the human disease alimentary toxic aleukia. Because of concerns about DON, the United States FDA has issued advisory levels of 1 ppm for wheat products for human consumption, 5 ppmfor grain products for most animal feeds and 10 ppm for grain products for cattle feed. The European Union has limits for DON that range from less than 0.2 ppm in processed cereal-based foods for infants and young children to less than 1.75 ppm for unprocessed wheat.
Using monoclonal antibody-based affinity chromatography, DONtest™ HPLC is an HPLC-only test for the detection of DON using immunoaffinity columns. Performed in less than 10 minutes, DONtest HPLC can measure levels of DON as low as 0.04 ppm and as high as 5 ppm.
With a total volume of 3 mL, compared to the total of 1mL in VICAM’s regular columns, DONtest WB allows for a faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.
Detection limit | 0.04 ppm |
Storage temperature|2 to 8°C(*)
(*) Freezing may damage the affinity columns