Clean-Up QAX (Quaternary Amine) unendcapped 500mg/10mL, 50 pkg

SAX is a strong anion exchange sorbent ideally suited for the extraction of compounds such as carboxylic acids, which may not retain effectively on weak anion exchange sorbents. Can have chloride, hydroxide, acetate or formate counter ion.

General method for column with 100mg of sorbent

Condition

1 mL Methanol

Equilibrate

1 mL Water pH 6–7

Load

Pretreated sample at pH 6–7

Wash 1

1 mL 25mM Ammonium Acetate Buffer, pH 6–7

Wash 2

1 mL Methanol, dry under vacuum for 2–5 min

Elute any acid

1 mL 5% NH4OH in Methanol

Elute weak acids

1 mL 5% Formic acid in Methanol

Recommended applications:

Caffeine, Organic acids, Saccharin